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* * * Catch a big tarpon, and you'll be a fan forever
Claire Wood and Donna Beaudreaux, great grandmother Charlene Thomas and his aunts and uncles
The first vector in both cases was based on the pDG461 plasmid (AddGene, Watertown, MA, USA) and contained a gene encoding Cas9(D10A), fused via a T2A sequence to an eGFP , as well as two specific gRNAs ( Supplementary Table 1 )
Those lucky enough to live in the tropics have a much wider choice as they can basically have any of the commonly sold tropical fish available in the aquarium trade
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